Diafiltration for Desalting or Buffer Exchange
نویسنده
چکیده
Diafiltration is an ultrafiltration membrane technique for completely removing, replacing, or lowering the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids, and other biomolecules. The process selectively uses permeable (porous) membrane filters to separate the components of solutions and suspensions based on their molecular size. Smaller molecules such as salts, solvents, and water pass freely through the ultrafiltration membrane, which retains the larger molecules.
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